pA2 online
Copyright 2004 The British Pharmacological Society

066P GKT, University of London
Winter Meeting December 2003

A computer simulation of experiments on the human eye to teach autonomic pharmacology

D. G. Dewhurst, M. J. Lew, J. Ziogas, & 1S. Cromar.1College of Medicine & Veterinary Medicine, University of Edinburgh, 15 George Square, Edinburgh EH8 9XD, UK & Dept. of Pharmacology, University of Melbourne

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Dewhurst DG
Lew MJ
Ziogas J
Cromar S

This program is based on a number of virtual patients with medical conditions which result in abnormal pupillary reflexes. It is designed to allow students to investigate the effects on the pupil diameter of the human eye of a physiological stimulus (change in light intensity) and a number of pharmacological agents which should help to suggest the underlying cause of the condition. The normal response to a change in light intensity is controlled by the autonomic nervous system and the program therefore provides a useful model for investigating autonomic pharmacology. Some of the agents could be administered externally to the real eye but others would not be safe.

The 'Tutorial' section describes, using text and animated schematic graphics, the sympathetic and parasympathetic control of pupil diameter and how pupil diameter changes in response to a change in ambient light intensity. The 'Student Exercise' section provides information on how to work through the investigation on a virtual patient using a 'normal' patient as an illustration. Thus students have the opportunity to investigate how the normal pupil will respond to a change in ambient light intensity, investigate the blink reflex and text the action of a number of pharmacological agents. They are encouraged to measure the pupil diameter (using an on-screen cross-hair cursor) at a range of light intensities and to observe, for each eye, the speed with which the pupil diameter changes. They can also investigate the action of a number of agents applied topically to the eye (single dose, enough for a large, but not maximal, response in eyes that are responsive) and record their observations on an on-screen chart. The agents available (atropine, pilocarpine, physostigmine, phenylephrine, cocaine, and amphetamine) all affect neurotransmission at the postganglionic sympathetic and parasympathetic synapses and have little effect on ganglionic transmission. There is also a 'washout' facility which instantly removes the applied drugs whereas in the real situation several hours might be required for some of the drug effects to be reversed.

The 'Simulation' section contains four virtual patients each suffering from a medical condition which results in an abnormal pupillary reflex in one eye (normal but with reddening of the eye and physiological anischoria; Horner's syndrome (pre-ganglionic); Horner's syndrome (postganglionic); partial parasympathectomy). Students first take measurements of the response to a change in light intensity which should give a clue to the underlying problem. They can then investigate this further by choosing two of the drugs from the list and observing their effects - that is sufficient to test the best hypothesis for each patient. To confirm their diagnosis students can then choose to administer one more agent after which they will be expected to select a diagnosis from the list.

The authors would like to acknowledge the support of The Lord Dowding Fund in the development of this program.